- Bacterial vaginosis
Bacterial vaginosis Classification and external resources
Micrograph of bacterial vaginosis — squamous cells of the cervix covered with rod-shaped bacteria, Gardnerella vaginalis (arrows).
ICD-10 B96, N76 ICD-9 616.1 MeSH D016585
Bacterial vaginosis (BV) or less commonly vaginal bacteriosis is a disease of the vagina caused by bacteria. The U.S. Centers for Disease Control and Prevention (CDC) states it is not clear what role sexual activity plays in the development. However, it is known that BV is associated with having a new sex partner or having multiple sex partners.   BV is caused by an imbalance of naturally occurring bacterial flora and is often confused with yeast infection (candidiasis), or infection with Trichomonas vaginalis (trichomoniasis), which are not caused by bacteria.
Symptoms and signs
The most common symptom of BV is an abnormal homogeneous off-white vaginal discharge (especially after sex) with an unpleasant smell. This malodorous discharge coats the walls of the vagina, and is usually without irritation, pain or erythema. By contrast, the normal vaginal discharge will vary in consistency and amount throughout the menstrual cycle and is at its clearest about 2 weeks before the period starts.
A healthy vagina normally contains many microorganisms; some of the common ones are Lactobacillus crispatus and Lactobacillus jensenii. Lactobacilli, particularly hydrogen peroxide-producing species, appear to help prevent other vaginal microorganisms from multiplying to a level where they cause symptoms. The microorganisms involved in BV are very diverse, but include Gardnerella vaginalis, Mobiluncus, Bacteroides, and Mycoplasma. A change in normal bacterial flora including the reduction of lactobacillus, which may be due to the use of antibiotics or pH imbalance, allows more resistant bacteria to gain a foothold and multiply.
Although BV can be associated with sexual activity, there is no clear evidence of sexual transmission.[non-primary source needed] It is possible for sexually inactive persons to get infected with bacterial vaginosis. Rather, BV is a disordering of the chemical and biological balance of the normal flora. Recent research is exploring the link between sexual partner treatment and eradication of recurrent cases of BV. Pregnant women and women with sexually transmitted infections are especially at risk for getting this infection. Bacterial vaginosis may sometimes affect women after menopause. A 2005 study by researchers at Ghent University in Belgium showed that subclinical iron deficiency (anemia) was a strong predictor of bacterial vaginosis in pregnant women. A longitudinal study published in February 2006 in the American Journal of Obstetrics and Gynecology showed a link between psychosocial stress and bacterial vaginosis independent of other risk factors.[clarification needed]
To make a diagnosis of bacterial vaginosis, a swab from inside the vagina should be obtained. These swabs should be tested for:
- A characteristic "fishy" odor on wet mount. This test, called the whiff test, is performed by adding a small amount of potassium hydroxide to a microscopic slide containing the vaginal discharge. A characteristic fishy odor is considered a positive whiff test and is suggestive of bacterial vaginosis.
- Loss of acidity. To control bacterial growth, the vagina is normally slightly acidic with a pH of 3.8–4.2. A swab of the discharge is put onto litmus paper to check its acidity. A pH greater than 4.5 is considered alkaline and is suggestive of bacterial vaginosis.
- The presence of clue cells on wet mount. Similar to the whiff test, the test for clue cells is performed by placing a drop of sodium chloride solution on a slide containing vaginal discharge. If present, clue cells can be visualized under a microscope. They are so-named because they give a clue to the reason behind the discharge. These are epithelial cells that are coated with bacteria.
Differential diagnosis for bacterial vaginosis includes the following:
- Normal discharge.
- Candidiasis (thrush, or a yeast infection).
- Trichomoniasis, an infection caused by Trichomonas vaginalis.
In clinical practice
In clinical practice BV is diagnosed using the Amsel criteria:
- Thin, white, yellow, homogeneous discharge
- Clue cells on microscopy
- pH of vaginal fluid >4.5
- Release of a fishy odor on adding alkali—10% potassium hydroxide (KOH) solution.
At least three of the four criteria should be present for a confirmed diagnosis.
- Grade 1 (Normal): Lactobacillus morphotypes predominate.
- Grade 2 (Intermediate): Mixed flora with some Lactobacilli present, but Gardnerella or Mobiluncus morphotypes also present.
- Grade 3 (Bacterial Vaginosis): Predominantly Gardnerella and/or Mobiluncus morphotypes. Few or absent Lactobacilli. (Hay et al., 1994)
What this technique loses in interobserver reliability, it makes up in ease and speed of use.
The standards for research are the Nugent Criteria. In this scale, a score of 0-10 is generated from combining three other scores. This method is time consuming and requires trained staff, but it has high interobserver reliability. The scores are as follows:
- 0–3 is considered negative for BV
- 4–6 is considered intermediate
- 7+ is considered indicative of BV.
At least 10–20 high power (1000× oil immersion) fields are counted and an average determined.
Lactobacillus morphotypes — average per high powered (1000× oil immersion) field. View multiple fields.
Curved Gram variable rods — average per high powered (1000× oil immersion) field. View multiple fields (note that this factor is less important — scores of only 0–2 are possible)
- Score 0 for >30
- Score 1 for 15–30
- Score 2 for 14
- Score 3 for <1 (this is an average, so results can be >0, yet <1)
- Score 4 for 0
- Score 0 for 0
- Score 1 for <1 (this is an average, so results can be >0, yet <1)
- Score 2 for 1–4
- Score 3 for 5–30
- Score 4 for >30
- Score 0 for 0
- Score 1 for <5
- Score 2 for 5+
A recent study  compared the Gram stain using the Nugent criteria and the DNA hybridization test Affirm VPIII in diagnosing BV. The Affirm VPIII test detected Gardnerella in 107 (93.0%) of 115 vaginal specimens positive for BV diagnosed by Gram stain. The Affirm VPIII test has a sensitivity of 87.7% and specificity of 96% and may be used for the rapid diagnosis of BV in symptomatic women.
The usual medical regimen for treatment is the antibiotic Metronidazole (500 mg twice a day, once every 12 hours) for 7 days. A one-time 2g dose is no longer recommended by the CDC because of low efficacy. Extended release metronidazole is an alternative recommendation.
In contrast to some other infectious diseases affecting the female genitals, according to some sources, treatment of the sexual partners is not necessarily recommended.
Although previously considered a mere nuisance infection, untreated bacterial vaginosis may cause serious complications, such as increased susceptibility to sexually transmitted infections including HIV, and may present other complications for pregnant women.
It is estimated that 1 in 3 women will develop the condition at some point in their lives.
- Non-specific urethritis
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- ^ "Bacterial Vaginosis – CDC Fact Sheet". Centers for Disease Control and Prevention. 1 September 2010. http://www.cdc.gov/std/bv/stdfact-bacterial-vaginosis.htm. Retrieved 11 November 2011.
- ^ a b c "National guideline for the management of bacterial vaginosis (2006)". Clinical Effectieness Group, British Association for Sexual Health and HIV (BASHH). http://www.guideline.gov/summary/summary.aspx?doc_id=11602.
- ^ Terri Warren, RN (2010). "Is It a Yeast Infection?". http://women.webmd.com/features/is-it-yeast-infection. Retrieved 2011-02-23.
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- ^ http://www.fda.gov/downloads/Drugs/GuidanceComplianceRegulatoryInformation/Guidances/ucm070969.pdf
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- ^ Nansel TR, Riggs MA, Yu KF, Andrews WW, Schwebke JR, Klebanoff MA (February 2006). "The association of psychosocial stress and bacterial vaginosis in a longitudinal cohort". Am. J. Obstet. Gynecol. 194 (2): 381–6. doi:10.1016/j.ajog.2005.07.047. PMC 2367104. PMID 16458633. http://linkinghub.elsevier.com/retrieve/pii/S0002-9378(05)01131-2.
- ^ a b Amsel R, Totten PA, Spiegel CA, Chen KC, Eschenbach D, Holmes KK (1983). "Nonspecific vaginitis. Diagnostic criteria and microbial and epidemiologic associations". Am. J. Med. 74 (1): 14–22. doi:10.1016/0002-9343(83)91112-9. PMID 6600371.
- ^ Ison, CA; Hay, PE (2002). "Validation of a simplified grading of Gram stained vaginal smears for use in genitourinary medicine clinics". Sex Transm Infect 78 (6): 413–5. doi:10.1136/sti.78.6.413. PMC 1758337. PMID 12473800. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1758337.
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- ^ a b Oduyebo OO, Anorlu RI, Ogunsola FT (2009). Oduyebo, Oyinlola O. ed. "The effects of antimicrobial therapy on bacterial vaginosis in non-pregnant women". Cochrane Database Syst Rev (3): CD006055. doi:10.1002/14651858.CD006055.pub2. PMID 19588379.
- ^ http://www.cdc.gov/std/treatment/2006/vaginal-discharge.htm
- ^ Potter J (November 1999). "Should sexual partners of women with bacterial vaginosis receive treatment?". Br J Gen Pract 49 (448): 913–8. PMC 1313567. PMID 10818662. http://openurl.ingenta.com/content/nlm?genre=article&issn=0960-1643&volume=49&issue=448&spage=913&aulast=Potter.
- ^ Senok AC, Verstraelen H, Temmerman M, Botta GA (2009). Senok, Abiola C. ed. "Probiotics for the treatment of bacterial vaginosis". Cochrane Database Syst Rev (4): CD006289. doi:10.1002/14651858.CD006289.pub2. PMID 19821358.
- ^ "STD Facts — Bacterial Vaginosis (BV)". CDC. http://www.cdc.gov/std/bv/STDFact-Bacterial-Vaginosis.htm#Complications. Retrieved 2007-12-04.
- ^ "The Family Planning Association". http://www.fpa.org.uk.
Female diseases of the pelvis and genitals (N70–N99, 614–629) InternalAdnexaVaginitis (Bacterial vaginosis, Atrophic vaginitis, Candidal vulvovaginitis) · Leukorrhea/Vaginal discharge · Hematocolpos/HydrocolposSexual dysfunction (Dyspareunia, Hypoactive sexual desire disorder, Sexual arousal disorder, Vaginismus)Other/generalPelvic inflammatory disease · Pelvic congestion syndrome External
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