ACES (buffer)

ACES (buffer)
IUPAC name 2-(carbamoylmethylamino)ethanesulfonic acid
Other names N-(2-Acetamido)-2-aminoethanesulfonic acid
Identifiers
CAS number	7365-82-4
PubChem	81832
ChemSpider	73843 Y
ChEBI	CHEBI:39062 Y
Jmol-3D images	Image 1 Image 2
SMILES C(CS(=O)(=O)O)NCC(=O)N O=S(=O)(O)CCNCC(=O)N
InChI InChI=1S/C4H10N2O4S/c5-4(7)3-6-1-2-11(8,9)10/h6H,1-3H2,(H2,5,7)(H,8,9,10) Y Key: DBXNUXBLKRLWFA-UHFFFAOYSA-N Y InChI=1/C4H10N2O4S/c5-4(7)3-6-1-2-11(8,9)10/h6H,1-3H2,(H2,5,7)(H,8,9,10) Key: DBXNUXBLKRLWFA-UHFFFAOYAH
Properties
Molecular formula	C4H10N2O4S
Molar mass	182.199
Y (buffer) (verify) (what is: Y/N?) Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa)
Infobox references

ACES is the common abbreviation for the compound N-(2-Acetamido)-2-aminoethanesulfonic acid.

ACES is one of Good's buffers developed in the 1960s to provide buffers with pH ranging from 6.15-8.35 for use in various applications. With a pK_a of 6.9, it is often used as a buffering agent in biological and biochemical research. It is a zwitterionic buffer with a useful buffering range of 6.1-7.5. The pioneering publication by Good and his co-workers described the synthesis and physical properties of ACES buffer.^[1]

Applications

ACES had been used to develop buffers for both agarose and polyacrylamide gel electrophoresis.^[2] ACES use in isoelectric focusing of proteins has also been documented.^[3] Use of ACES has been published in a protocol for the analysis of bacterial autolysins in a discontinuous SDS-PAGE system.^[4] Potential inhibition of ACES and other Good buffers has been investigated in γ-aminobutyric acid receptor binding to rat brain synaptic membranes.^[5]

References

1. ^ Good, N.E., "Hydrogen ion buffers for biological research." "Biochemistry", 5(2), 467-477.
2. ^ Liu, Q., et al., "pK-matched running buffers for gel electrophoresis." "Anal. Biochemistry.", 270(1):112-122.
3. ^ Alonso, A., "Human α-1-antitrypsin subtyping by hybrid isoelectric focusing in miniaturized polyacrylamide gel." "Electrophoresis", 9(2):65-73.
4. ^ Strating, H., and Clarke, A.J., "Differentiation of bacterial autolysins by zymogram analysis." "Anal. Biochem.", 291(1):149-154.
5. ^ Tunnicliff, G., and Smith, J.A., "Competitive inhibition of γ-aminobutyric acid receptor binding by N-2-hydroxyethylpiperazine-N'-2-ε-ethanesulfonic acid and related buffer." "J. Neurochem.", 36(3):1122-1126.