Thermus thermophilus

Taxobox
name = "Thermus thermophilus"
regnum = Bacteria
phylum = Deinococcus-Thermus
classis = Deinococci
ordo = Thermales
familia = Thermaceae
genus = "Thermus"
species = "T. thermophilus"
binomial = "Thermus thermophilus"

"Thermus thermophilus" is a gram negative eubacterium used in a range of biotechnological applications, including as a model organism for genetic manipulation, structural genomics, and systems biology. The bacterium is extremely thermophilic, with an optimal growth temperature of about 65ºC. "Thermus thermophilus" was originally isolated from a thermal vent environment in Japan by Tairo Oshima and Kazutomo Imahori. [cite journal
journal=Int J Syst Bacteriol.
year=1974
volume=24
pages=102–112
title=Description of "Thermus thermophilus" (Yoshida and Oshima) comb. nov., a nonsporulating thermophilic bacterium from Japanese thermal spa.
author=Oshima & Imahori] "T. thermophilus" is classified into several strains, in which HB8 and HB27 are most common and their genome analyses were independently completed in 2004. [cite journal
journal=Nat Biotechnol.
year=2004
month=May
volume=22
issue=5
pages=547–53
title=The genome sequence of the extreme thermophile "Thermus thermophilus"
author=Henne A et al.
pmid=15064768
doi=10.1038/nbt956]

Biotechnological applications of "Thermus thermophilus" enzymes

* rTth DNA polymerase is a recombinant thermostable DNA polymerase derived from "Thermus thermophilus", with optimal activity at 70-80ºC, used in some PCR applications. The enzyme possesses efficient reverse transcriptase activity in the presence of manganese.

External links

* [http://www.tigr.org/tigr-scripts/CMR2/GenomePage3.spl?database=nttt02 "Thermus thermophilus" HB27 genome page.]
* [http://www.tigr.org/tigr-scripts/CMR2/GenomePage3.spl?database=nttt03 "Thermus thermophilus" HB8 genome page.]
* [http://www.thermus.org/e_index.htm Structural-biological whole cell project of "Thermus thermophilus" HB8.]

References