Protein methods

Protein methods

Protein methods are the techniques used to study proteins.

List of Protein Methods

There are genetic methods for studying proteins, methods for detecting proteins, methods for isolating and purifying proteins and other methods for characterizing the structure and function of proteins, often requiring that the protein first be purified.

Genetic methods

*conceptual translation- many proteins are never directly sequenced, but their sequence of amino acids is known by "conceptual translation" of a known mRNA sequence. See Genetic code.
*site-directed mutagenesis allows new variants of proteins to be produced and tested for how structural changes alter protein function.
**insertion of protein tags such as the His-tag. See also: Green fluorescent protein.
*evolutionary; analysis of sequence changes in different species using software such as BLAST.
*Proteins that are involved in human diseases can be identified by matching alleles to disease and other phenotypes using methods such as calculation of LOD scores.

Detecting proteins

*microscopy, Protein immunostaining
*immunoprecipitation, Protein immunoprecipitation
*Western blot
*Spectrophotometry
*Enzyme assay

Protein purification

* Protein Isolation
**chromatography methods
* Protein Extraction and Solubilization
* Protein Concentration Determination Methods, Bradford protein assay
* Concentrating Protein Solutions
*Gel electrophoresis
** Gel Electrophoresis Under denaturing conditions
** Gel Electrophoresis Under non-denaturing conditions
** 2D Gel Electrophoresis
*Electrofocusing

Protein structures

*X-ray crystallography
*Protein NMR

Protein-DNA Interactions

*ChIP-on-chip
*Chip-Sequencing

Other methods

* Hydrogen-deuterium exchange
* Mass spectrometry
* Molecular dynamics
* Protein structure prediction
* Protein sequencing
* Protein structural alignment
* Protein ontology
* Peptide mass fingerprinting
* Ligand binding assay
*metabolic labeling
**heavy isotope labeling
**radioactive isotope labeling

References

*"Protein Methods", 2nd Edition by Daniel M. Bollag, Michael D. Rozycki and Stuart J. Edelstein (1996) Published by Wiley Pblishers ISBN 0-471-11837-0.


Wikimedia Foundation. 2010.

Игры ⚽ Поможем написать реферат

Look at other dictionaries:

  • Protein purification — is a series of processes intended to isolate a single type of protein from a complex mixture. Protein purification is vital for the characterisation of the function, structure and interactions of the protein of interest. The starting material is… …   Wikipedia

  • Protein adulteration in the People's Republic of China — refers to the adulteration and contamination of several food and feed ingredients with inexpensive melamine and other compounds such as cyanuric acid, ammeline and ammelide. These adulterants can be used to inflate the apparent protein content of …   Wikipedia

  • Protein folding — Protein thermodynamics redirects here. For the thermodynamics of reactions catalyzed by proteins, see Enzyme. Protein before and after folding. Protein folding is the process by which a protein structure assumes its functional shape or… …   Wikipedia

  • Protein (disambiguation) — Protein is a class of biomolecules composed of amino acid chains.Protein may also refer to:Biochemistry* Antifreeze protein, class of polypeptides produced by certain fish, vertebrates, plants, fungi and bacteria * Conjugated protein, protein… …   Wikipedia

  • Protein domain — Pyruvate kinase, a protein from three domains (PDB 1pkn) A protein domain is a part of protein sequence and structure that can evolve, function, and exist independently of the rest of the protein chain. Each domain forms a compact three… …   Wikipedia

  • Methods to investigate protein–protein interactions — There are many methods to investigate protein–protein interactions. Each of the approaches has its own strengths and weaknesses, especially with regard to the sensitivity and specificity of the method. A high sensitivity means that many of the… …   Wikipedia

  • Protein (nutrient) — This article is about protein as a nutrient. For protein as a class of molecule, see protein. For other uses, see bodybuilding supplement. Amino acids are the building blocks of protein. Proteins are polymer chains made of amino acids linked… …   Wikipedia

  • Protein targeting — This article deals with protein targeting in eukaryotes except where noted. Protein targeting or protein sorting is the mechanism by which a cell transports proteins to the appropriate positions in the cell or outside of it. Sorting targets can… …   Wikipedia

  • Methods in enzymology (collection d'ouvrages) — Methods in Enzymology Methods in enzymology est une série d ouvrages éditée depuis le milieu des années 1950 par les éditions Academic Press / Elsevier. Cette colossale collection représente la plus importante somme de connaissance dans le… …   Wikipédia en Français

  • Protein structure prediction — is one of the most important goals pursued by bioinformatics and theoretical chemistry. Its aim is the prediction of the three dimensional structure of proteins from their amino acid sequences, sometimes including additional relevant information… …   Wikipedia

Share the article and excerpts

Direct link
… Do a right-click on the link above
and select “Copy Link”