ortho-Nitrophenyl-β-galactoside

ortho-Nitrophenyl-β-galactoside
ortho-Nitrophenyl-β-galactoside
Identifiers
CAS number 19710-96-4
PubChem 123646
MeSH 2-nitrophenylgalactoside
Jmol-3D images Image 1
Properties
Molecular formula C12H15NO8
Molar mass 301.249
Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa)
Infobox references

ortho-Nitrophenyl-β-galactoside (ONPG) is a colorimetric and spectrophotometric substrate for detection of beta-galactosidase activity. This compound is normally colorless. However if β-galactosidase is present, it hydrolyzes the ONPG molecule into galactose and ortho-nitrophenol. The latter compound has a yellow color that can be used to check for enzyme activity by means of a colorimetric assay (at 420 nm wavelength). β-galactosidase is required for lactose utilization, so the intensity of the color produced can be used as a measure of the enzymatic rate.

Though ONPG mimics lactose and is hydrolyzed by β-galactosidase, it is unable to act as an inducer for the lac operon. Without another lactose analog that can act as an inducer, such as IPTG, β-galactosidase will not be transcribed and ONPG will not be hydrolyzed.



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  • Ortho-Nitrophenyl-β-galactoside — Chembox new ImageFile = ONPG structure.png ImageSize = IUPACName = ( 2R,3R,4S,5R ) 2 (Hydroxymethyl) 6 (2 nitrophenoxy)oxane 3,4,5 triol OtherNames = 2 Nitrophenylgalactoside Abbreviations = ONPG Section1 = Chembox Identifiers CASNo = 19710 96 4… …   Wikipedia

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  • 19710-96-4 — Ortho nitrophenyl β galactoside Ortho nitrophenyl β galactoside Figure 1 : Formule de l ONPG. Général No CAS …   Wikipédia en Français

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