Matrix-assisted laser desorption electrospray ionization

Matrix-assisted laser desorption electrospray ionization
Schematic of a typical MALDESI setup

Matrix-assisted laser desorption electrospray ionization (MALDESI) is a method for creating ions that can be used for chemical analysis in a mass spectrometer.[1] It is related to desorption electrospray ionization (DESI) and is a combination of matrix-assisted laser desorption ionization (MALDI) and electrospray ionization.

How it works

In MALDESI, a pulsed laser is fired at a sample to desorb or ablate material as particles, clusters or molecules. The sample is typically at atmospheric pressure and ambient temperature. As with MALDI, the sample to be analyzed is mixed with a matrix material that absorbs the laser energy and aids in the laser removal of material. The neutral material interacts with charged droplets in the electrospray plume to form ions that are sampled by a mass spectrometer.

Related techniques

Some samples form ions without the need for a matrix. The technique called electrospray-assisted laser desorption/ionization (ELDI) uses an ultraviolet laser to form ions by irradiating the sample sample directly for ion formation through interaction with the electrospray plume.[2] The infrared laser version of ELDI has been called laser ablation electrospray ionization (LAESI)[3] or, alternately, infrared laser assisted desorption electrospray ionization (IR-LADESI).[4][5]

In early versions of thermospray, an infrared laser was directed at a liquid spray tip to heat the sample and aid in ionization.[6] Desorption atmospheric pressure photoionization (DAPPI) uses a jet of heated solvent for desorption and ultraviolet light for photoionization.[7]

References

  1. ^ Sampson JS, Hawkridge AM, Muddiman DC (2006). "Generation and detection of multiply charged peptides and proteins by matrix-assisted laser desorption electrospray ionization (MALDESI) Fourier transform ion cyclotron resonance mass spectrometry". J. Am. Soc. Mass Spectrom. 17 (12): 1712–6. doi:10.1016/j.jasms.2006.08.003. PMID 16952462. 
  2. ^ Shiea J, Huang MZ, Hsu HJ, Lee CY, Yuan CH, Beech I, Sunner J (2005). "Electrospray-assisted laser desorption/ionization mass spectrometry for direct ambient analysis of solids". Rapid Commun. Mass Spectrom. 19 (24): 3701–4. doi:10.1002/rcm.2243. PMID 16299699. 
  3. ^ Nemes P, Vertes A (2007). "Laser Ablation Electrospray Ionization for Atmospheric Pressure, in Vivo, and Imaging Mass Spectrometry". Analytical Chemistry 79 (21): 8098–106. doi:10.1021/ac071181r. PMID 17900146. 
  4. ^ Dong, Jianan; Y. Rezenom and K. Murray (June 4, 2007). "Aerosol Desorption Electrospray Ionization". 55th ASMS Meeting. Indianapolis, IN: American Society for Mass Spectrometry. pp. MP006 
  5. ^ Rezenom, Yh; Dong, J; Murray, Kk (February 2008). "Infrared laser-assisted desorption electrospray ionization mass spectrometry". The Analyst 133 (2): 226–32. doi:10.1039/b715146b. PMID 18227946 
  6. ^ Blakley, C. R.; Carmody, J. J.; Vestal, M. L. "Liquid Chromatograph-Mass Spectrometer for Analysis of Nonvolatile Samples" Analytical Chemistry 1980, 52, 1636-1641.
  7. ^ Haapala M, Pól J, Saarela V et al. (2007). "Desorption Atmospheric Pressure Photoionization". Analytical Chemistry 79 (20): 7867–7872. doi:10.1021/ac071152g. PMID 17803282. 
v · d · eMass spectrometry
Mass • m/z • Mass spectrum • MS software • Acronyms
Ion source
EI  • CI  • IA  • FAB  • FD  • MALDI  • APCI  • APLI  • ESI  • DESI  • GD  • ICP  • MIP  • SS  • TI  • TS  • DART  • DAPPI
Mass analyzer
Detector
MS combination
MS/MS  • QqQ  • Hybrid MS  • GC/MS  • LC/MS  • IMS/MS
Fragmentation
CID  • IRMPD  • BIRD  • ECD  • ETD  • EDD  • NETD  • SID  • HCD

Wikimedia Foundation. 2010.

Игры ⚽ Поможем написать реферат

Look at other dictionaries:

  • Matrix-assisted laser desorption/ionization — MALDI TOF mass spectrometer Matrix assisted laser desorption/ionization (MALDI) is a soft ionization technique used in mass spectrometry, allowing the analysis of biomolecules (biopolymers such as DNA, proteins, peptides and sugars) and large… …   Wikipedia

  • Desorption-ionisation laser assistee par matrice — Désorption ionisation laser assistée par matrice Spectromètre de masse MALDI TOF La désorption ionisation laser assistée par matrice (en anglais Matrix Assisted Laser Desorption Ionisation ou MALDI) est une technique d ionisation douce utilisée… …   Wikipédia en Français

  • Désorption-Ionisation Laser Assistée Par Matrice — Spectromètre de masse MALDI TOF La désorption ionisation laser assistée par matrice (en anglais Matrix Assisted Laser Desorption Ionisation ou MALDI) est une technique d ionisation douce utilisée en spectrométrie de masse, permettant l analyse de …   Wikipédia en Français

  • Laser spray — is a new liquid chromatography/mass spectrometry interface. Explosive vaporization and mist formation occur when an aqueous solution effusing out from the tip of the stainless steel capillary is irradiated from the opposite side of the capillary… …   Wikipedia

  • Désorption-ionisation laser assistée par matrice — Spectromètre de masse MALDI TOF La désorption ionisation laser assistée par matrice (en anglais Matrix Assisted Laser Desorption Ionisation ou MALDI) est une technique d ionisation douce utilisée en spectrométrie de masse, permettant l analyse de …   Wikipédia en Français

  • MALDI — Désorption ionisation laser assistée par matrice Spectromètre de masse MALDI TOF La désorption ionisation laser assistée par matrice (en anglais Matrix Assisted Laser Desorption Ionisation ou MALDI) est une technique d ionisation douce utilisée… …   Wikipédia en Français

  • Mass spectrometry — (MS) is an analytical technique that measures the mass to charge ratio of charged particles.[1] It is used for determining masses of particles, for determining the elemental composition of a sample or molecule, and for elucidating the chemical… …   Wikipedia

  • History of mass spectrometry — The history of mass spectrometry dates back more than one hundred years and has its roots in physical and chemical studies regarding the nature of matter. The study of gas discharges in the mid 19th century led to the discovery of anode and… …   Wikipedia

  • Ion source — An ion source is an electro magnetic device that is used to create charged particles. These are used primarily within mass spectrometers or particle accelerators.Mass spectrometry In mass spectrometry, an ion source is a piece of equipment used… …   Wikipedia

  • Time-of-flight mass spectrometry — (TOFMS) is a method of mass spectrometry in which ions are accelerated by an electric field of known strength. [Stephens, W. E., [http://link.aps.org/abstract/PR/v69/p674/s2 A Pulsed Mass Spectrometer with Time Dispersion] Phys. Rev. , 1946, 69 …   Wikipedia

Share the article and excerpts

Direct link
… Do a right-click on the link above
and select “Copy Link”