DAT or Dat may refer to:
- Direct agglutination test, any test that uses whole organisms as a means of looking for serum antibody
- Direct antiglobulin test, one of two Coombs tests
- Dopamine transporter or dopamine active transporter, a membrane-spanning protein that pumps the neurotransmitter dopamine out of the synapse and into its vesicles for storage until later use
- .dat, a common file format
- Data Access Technology, an American telecommunications company
- Dial-a-truck, or DAT - the first electronic freight posting/searching load board service
- Digital Audio Tape, a signal recording and playback medium developed by Sony and introduced in 1987
- Distributed active transformer, low voltage transistors used to generate radio frequencies
- Double acting tanker, a type of icebreaking merchant ship designed to run ahead in open water and astern in ice
- Dynamic Address Translation, a term for translating virtual addresses to real address in a virtual memory computer system
- DAT Danish Air Transport, an airline based in Vamdrup, Denmark
- Dat Phan (born 1975), a Vietnamese-American stand-up comedian
- Day After Tomorrow (band), a 3-member J-pop band under the Avex label
- Delta Air Transport, an airline founded in 1967 and based in Belgium
- Dental Admission Test, a multiple-choice standardized exam taken by potential dental school students in the United States and Canada
- Design and Technology, school subject in primary and secondary school
- Desk appearance ticket, an order to appear in county court for an arraignment
- Disaster Action Team, the local disaster response unit of the American Red Cross
- Doctor of Athletic Training, Advanced Clinical Doctorate in Athletic Training reserved for practicing athletic trainers. Currently the only DAT exists at the University of Idaho. The DAT is an advance practice doctorate.
- Drug action teams, multi-agency partnerships created by the 1995 United Kingdom national drug strategy
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