- blue-white colour selection
Method for identifying bacterial clones containing plasmids with inserts. Many modern vectors have their polycloning site within a part of the LacZ gene encoding beta-galactosidase, which provides a -complementation in an appropriate mutant E. coli strain. This means that a re-ligated (empty) vector will produce blue colonies when grown on plates containing IPTG and Xgal, but colonies with a substantial insert in their plasmid\'s polycloning site are unable to produce functional beta-galactosidase, and so produce white colonies.
Dictionary of molecular biology. 2004.
Look at other dictionaries:
colour — /kul euhr/, n., adj. v.t., v.i. Chiefly Brit. color. Usage. See or1. * * * I Aspect of any object that may be described in terms of hue, brightness, and saturation. It is associated with the visible wavelengths of electromagnetic radiation, which … Universalium
White people — Whites redirects here. For other uses, see White (disambiguation). Race … Wikipedia
Blue (university sport) — A Blue is an award earned by sportsmen and women at a university and some schools for competition at the highest level. The awarding of Blues began at Oxford and Cambridge Universities. It is found at British, Australian and New Zealand… … Wikipedia
Colour cast — A colour cast is a tint of a particular colour, usually unwanted, which affects the whole of a photographic image evenly. Certain types of light can cause film and digital cameras to have a colour cast. In general, the human eye does not notice… … Wikipedia
Blue plaque — In the United Kingdom, a blue plaque is a permanent sign installed in a public place to commemorate a link between that location and a famous person or event. It serves as a historic marker.English Heritage schemePerhaps the best known blue… … Wikipedia
Colonel White — Captain Scarlet and the Mysterons character Created by Gerry Anderson Voiced by Donald Gray (Captain Scarlet and the Mysterons) Mike Hayley (Gerry Anderson s New Captain Scarlet) … Wikipedia
beta-galactosidase — (= b galactosidase) Enzyme (EC 22.214.171.124) encoded by the LacZ gene, that is widely used as a reporter gene, as a variety of coloured or fluorescent compounds can be produced from appropriate substrates (typically Xgal, that produces a blue colour) … Dictionary of molecular biology
Xgal — (= 5 bromo 4 chloro 3 indolyl b D galactoside) Chromogenic substrate for the enzyme beta galactosidase, yielding an intense blue colour. Very widely used in blue white colour selection of bacteria containing recombinant plasmids, and in… … Dictionary of molecular biology
heredity — /heuh red i tee/, n., pl. heredities. Biol. 1. the transmission of genetic characters from parents to offspring: it is dependent upon the segregation and recombination of genes during meiosis and fertilization and results in the genesis of a new… … Universalium
arts, East Asian — Introduction music and visual and performing arts of China, Korea, and Japan. The literatures of these countries are covered in the articles Chinese literature, Korean literature, and Japanese literature. Some studies of East Asia… … Universalium