blotting
General term for the transfer of protein, RNA or DNA molecules from a relatively thick acrylamide or agarose gel to a paper-like membrane (usually nylon or nitrocellulose) by capillarity or an electric field, preserving the spatial arrangment. Once on the membrane, the molecules are immobilized, typically by baking or by ultraviolet irradiation, and can then be detected at high sensitivity by hybridization (in the case of DNA and RNA), or antibody labelling (in the case of protein). RNA blots are called Northern blots; DNA blots, Southern; protein blots, Western. See also dot and slot blots.

Dictionary of molecular biology. 2004.

Look at other dictionaries:

  • blotting — mid 15c., verbal noun from prp. of BLOT (Cf. blot) (v.). Blotting paper is recorded from 1510s …   Etymology dictionary

  • blotting — blotting; im·mu·no·blotting; …   English syllables

  • blotting — blotting. См. блоттинг. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • Blotting — Als Blotting oder Blotten bezeichnet man in der Molekularbiologie ein Verfahren zum Transfer von DNA, RNA oder Proteinen auf eine Membran. Der Name leitet sich aus dem englischen „to blot“ (klecksen, beflecken, mit Löschpapier abtupfen) ab, was… …   Deutsch Wikipedia

  • Blotting — Blọt|ting [engl. blot = [be]klecksen, ablöschen]: eine molekularbiol. Nachweismethode, bei der z. B. elektrophoretisch getrennte DNA Fragmente – nach ↑ Denaturierung (1) innerhalb der Gelschicht – auf eine Cellulosenitrat Matrix übertragen… …   Universal-Lexikon

  • Blotting — Blot Blot, v. t. [imp. & p. p. {Blotted}; p. pr. & vb. n. {Blotting}.] [Cf. Dan. plette. See 3d {Blot}.] [1913 Webster] 1. To spot, stain, or bespatter, as with ink. [1913 Webster] The brief was writ and blotted all with gore. Gascoigne. [1913… …   The Collaborative International Dictionary of English

  • blotting — blot·ting (blotґing) soaking up with or transferring to absorbent material. For specific techniques using blotting to analyze nucleic acids and proteins, see entries under blot …   Medical dictionary

  • Blotting — Промокание; Стирание, уничтожение написанного; Загрязнение (помарками, пятнами) …   Краткий толковый словарь по полиграфии

  • blotting — adj. obscuring; absorbing, soaking up n. erasing, eliminating; blotching, staining blÉ’t n. stain, blemish; smear, speckle v. stain; absorb with blotter paper …   English contemporary dictionary

  • blotting — bottling …   Anagrams dictionary

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