RAPD
Variation of the polymerase chain reaction used to identify differentially expressed genes. mRNA from two different tissue samples is reverse transcribed, then amplified using short, intentionally nonspecific primers. The array of bands obtained from a series of such amplifications is run on a high resolution gel and compared with analogous arrays from different samples. Any bands unique to single samples are considered to be differentially expressed; they can be purified from the gel, and sequenced and used to clone the full-length cDNA. Similar in aim to subtractive hybridization. See also differential display PCR.

Dictionary of molecular biology. 2004.

Look at other dictionaries:

  • RAPD — stands for Random Amplification of Polymorphic DNA. It is a type of PCR reaction, but the segments of DNA that are amplified are random. The scientist performing RAPD creates several arbitrary, short primers (8 12 nucleotides), then proceeds with …   Wikipedia

  • RAPD — (sprich rapid) ist die Abkürzung für engl. randomly amplified polymorphic DNA, zu deutsch zufällig vervielfältigte polymorphe DNA. RAPD ist eine besondere Form der Polymerase Kettenreaktion. Die Methode wurde erstmals 1990 von Williams et al.… …   Deutsch Wikipedia

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  • RAPD — Abbreviation for rapid analysis of polymorphic DNA. * * * randomly amplified polymorphic deoxyribonucleic acid [DNA]; relative afferent pupillary defect …   Medical dictionary

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  • RAPD —   Random Amplified Polymorphic DNA; a technique in DNA analysis based on patterns obtained in electrophoresis after PCR amplification using randomly selected primers.   See also AFLP, RFLP …   Expanded glossary of Cycad terms

  • RAPD — • randomly amplified polymorphic deoxyribonucleic acid [DNA]; • relative afferent pupillary defect …   Dictionary of medical acronyms & abbreviations

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  • RAPD (disambiguation) — RAPD may refer to:* RAPD Random Amplification of Polymorphic DNA * Marcus Gunn pupil Relative Afferent Pupillary Defect …   Wikipedia

  • ДНК-маркер — Рис.1 Электрофорез ПЦР продуктов в агарозном геле и окрашенных бромистым этидием, полученных методом IRAP амплификации. Левая дорожка  ДНК известной длины (от 900 до 3000 пар нуклеотидов) …   Википедия

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