periodic acid-Schiff reaction


periodic acid-Schiff reaction
A method for staining carbohydrates: adjacent hydroxyl groups are oxidized to form aldehydes by periodic acid (HIO4 ) and these aldehyde groups react with Schiff\'s reagent (basic fuchsin decolorised by sulphurous acid) to give a purple colour. Used in histochemistry and in staining gels on which glycoproteins have been run.

Dictionary of molecular biology. 2004.

Look at other dictionaries:

  • periodic acid-Schiff reaction — (PAS reaction) a test for the presence of glycoproteins, polysaccharides, certain mucopolysaccharides, glycolipids, and certain fatty acids in tissue sections. The tissue is treated with periodic acid, followed by Schiff s reagent. A positive… …   Medical dictionary

  • periodic acid–Schiff reaction — PAS reaction a test for the presence of glycoproteins, polysaccharides, certain mucopolysaccharides, glycolipids, and certain fatty acids in tissue sections. The tissue is treated with periodic acid, followed by Schiff s reagent. A positive… …   The new mediacal dictionary

  • periodic acid–Schiff reaction — a tissue section is exposed to periodic acid, which oxidizes hydroxyl groups on adjacent carbon atoms or adjacent hydroxyl groups and amino groups to aldehydes, and then is stained with Schiff reagent, which forms an additional product with… …   Medical dictionary

  • Periodic acid-Schiff stain — Periodic acid Periodic acid Schiff (PAS) is a staining method used to detect glycogen and other polysaccharides in tissues. The reaction of periodic acid oxidizes the diol functional groups in glucose and other sugars, creating aldehydes that… …   Wikipedia

  • periodic acid-Schiff — shif adj relating to, being, or involving a reaction testing for polysaccharides and related substances in which tissue sections are treated with periodic acid and then Schiff s reagent with a reddish violet color indicating a positive test… …   Medical dictionary

  • Шик-Реакция, Pas-Реакция (Periodic Acid — Schiff (PAS) reaction) тест, позволяющий выявить наличие в тканях гликопротеинов, полисахаридов, некоторых мукополисахаридов, гликолипидов и ряда жирных кислот. Исследуемая ткань после воздействия на нее реактива Шиффа обрабатывается йодной… …   Медицинские термины

  • Schiff's reagent — See periodic acid Schiff reaction …   Dictionary of molecular biology

  • Schiff test — The Schiff test invented [ Eine neue Reihe organischen Diaminen H. Schiff Justus Liebigs Ann Chemie 140, 92 137 1866 ] and named after Hugo Schiff is a chemical test for the detection of aldehydes [ Histology, cell and tissue biology 5th Ed.,… …   Wikipedia

  • PAS reaction — periodic acid–Schiff reaction …   The new mediacal dictionary

  • PAS reaction — see periodic acid Schiff reaction * * * periodic acid–Schiff r …   Medical dictionary


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