PAP technique
(1) Colloquial abbreviation for Papanicolaou\'s stain,
(2) Peroxidase-antiperoxidase method for obtaining an enhanced peroxidase reaction to indicate antibody binding to antigen. In the first stage the material, eg. a section, is reacted with a specific antiserum (say rat) against the antigen. In the next stage a large excess of (say) rabbit anti-rat immunoglobulin is applied so that only one of the binding sites is bound to the first antibody. Then a rat antiperoxidase antiserum is bound to the second antibody\'s unfilled sites and finally peroxidase is added and binds to the third antiserum before the peroxidase is used to develop a colour reaction.

Dictionary of molecular biology. 2004.

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