nuclear run-on


nuclear run-on
(= nuclear run-off)
Strictly different, the two terms tend to be used interchangeably. A nuclear run-on assay is intended to identify the genes that were being transcribed at a particular instant; nuclei are rapidly isolated from cells, and incubated with labelled nucleotides. This gives a population of labelled RNAs that were being transcribed immediately before isolation. These can be studied directly; or (more commonly) used as a probe to identify corresponding cDNAs.

Dictionary of molecular biology. 2004.

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