nested primers


nested primers
Sets of primers for PCR so arranged that the second set to be used lie within the sequence amplified by the first set of primers and so on.

Dictionary of molecular biology. 2004.

Look at other dictionaries:

  • Nested polymerase chain reaction — A diagram illustrating the method of nested PCR. Nested polymerase chain reaction is a modification of polymerase chain reaction intended to reduce the contamination in products due to the amplification of unexpected primer binding sites.… …   Wikipedia

  • nested PCR — Variety of polymerase chain reaction, in which specificity is improved by using two sets of primers sequentially. An initial PCR is performed with the ‘outer’ primer pairs, then a small aliquot is used as a template for a second round of PCR with …   Dictionary of molecular biology

  • Primer Premier — Infobox Software name = Primer Premier caption = developer = Premier Biosoft latest release version = 5.0 latest release date = 6 May 2008 latest preview version = latest preview date = operating system = Windows platform = genre = Bioinformatics …   Wikipedia

  • Baby Gender Mentor — The Baby Gender Mentor packaging advertises a controversial 99.9% accuracy rate and a 48 hour turn around time. Baby Gender Mentor is the trade name of a controversial blood test designed for prenatal sex discernment, that is, to determine… …   Wikipedia

  • Enolase 2 — (gamma, neuronal) PDB rendering based on 1te6 …   Wikipedia

  • Polymerase chain reaction — PCR redirects here. For other uses, see PCR (disambiguation). A strip of eight PCR tubes, each containing a 100 μl reaction mixture The polymerase chain reaction (PCR) is a scientific technique in molecular biology to amplify a single or a… …   Wikipedia

  • Variants of PCR — This page assumes familiarity with the terms and components used in the Polymerase Chain Reaction (PCR) process. The versatility of PCR has led to a large number of variants: Contents 1 Basic modifications 2 Pretreatments and extensions 3 Other… …   Wikipedia

  • Bisulfite sequencing — Figure 1: Outline of bisulfite conversion of sample sequence of genomic DNA. Nucleotides in blue are unmethylated cytosines converted to uracils by bisulfite, while red nucleotides are 5 methylcytosines resistant to conversion …   Wikipedia

  • Polymerase chain reaction optimization — The polymerase chain reaction (PCR) is a commonly used molecular biology tool for amplifying DNA, and various techniques for PCR optimization have been developed by molecular biologists to improve PCR performance and minimize failure. Contents 1… …   Wikipedia

  • Overlap extension polymerase chain reaction — This page assumes familiarity with the terms and components used in polymerase chain reaction (PCR) process. The overlap extension polymerase chain reaction (or OE PCR) is a variant of PCR. It is also referred to as Splicing by overlap extension… …   Wikipedia


Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”

We are using cookies for the best presentation of our site. Continuing to use this site, you agree with this.