Maxam-Gilbert method


Maxam-Gilbert method
A method of DNA sequencing, based on the controlled degradation of a DNA fragment in a set of independent, nucleotide-specific reactions. The resulting fragments have characteristic sizes depending on the sequence of the template, that can be resolved on a sequencing gel. Although no longer the main protocol, Maxam-Gilbert sequencing still has advantages, eg. for oligonucleotides or covalently modified DNA. See also dideoxy sequencing.

Dictionary of molecular biology. 2004.

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  • Maxam-Gilbert method — Maxam Gilbert method. См. химический метод секвенирования ДНК. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • chemical method of DNA sequencing — chemical method of DNA sequencing. = Maxam Gilbert method (см.). (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • Allan Maxam — Infobox Scientist name = Allan Maxam box width = image width =150px caption = birth date = birth place = death date = death place = residence = citizenship = nationality = ethnicity = field = work institutions = alma mater = doctoral advisor =… …   Wikipedia

  • Walter Gilbert — Infobox Scientist name = Walter Gilbert birth date = birth date and age|1932|03|21 birth place = Boston, Massachusetts, U.S. residence = nationality = U.S. field = Biochemistry, physics work institutions = alma mater = doctoral advisor = doctoral …   Wikipedia

  • Walter Gilbert — (21 mars 1932) est un biochimiste, médecin, pionnier de la biologie moléculaire et entrepreneur américain. Il est colauréat avec Frederick Sanger de la moitié du prix Nobel de chimie de 1980 …   Wikipédia en Français

  • химический метод секвенирования ДНК — Maxam Gilbert method, chemical method of DNA sequencing метод Максама Гилберта, химический метод секвенирования ДНК. Один из наиболее распространенных методов определения последовательности нуклеотидов в молекулах ДНК: анализируемый фрагмент ДНК… …   Молекулярная биология и генетика. Толковый словарь.

  • recombinant DNA technology — Genetics. any of various techniques for separating and recombining segments of DNA or genes, often employing a restriction enzyme to cut a gene from a donor organism and inserting it into a plasmid or viral DNA for transplantation into a host… …   Universalium

  • DNA sequencing — ▪ genetics  technique used to determine the nucleotide sequence of DNA (deoxyribonucleic acid). The nucleotide sequence is the most fundamental level of knowledge of a gene or genome. It is the blueprint that contains the instructions for… …   Universalium

  • dideoxy sequencing — (= Sanger dideoxy sequencing) The most popular method of DNA sequence determination (cf. Maxam Gilbert method). Starting with single stranded template DNA, a short complementary primer is annealed, and extended by a DNA polymerase. The reaction… …   Dictionary of molecular biology

  • DNA sequence analysis — Determination of the nucleotide sequence of a length of DNA. Typically, this is performed by cloning the DNA of interest, so that enough can be prepared to allow the sequence to be determined, usually by the Sanger dideoxy sequencing method or… …   Dictionary of molecular biology


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