isoelectric focusing
Electrophoresis in a stabilized pH gradient. High-resolution method for separating molecules, especially proteins, that carry both positive and negative charges. Molecules migrate to the pH corresponding to their isoelectric point. The gradient is produced by electrophoresis of amphiphiles, heterogenous molecules giving a continuum of isoelectric points. Resolution is determined by the number of amphiphile species and the evenness of distribution of their isoelectric points.

Dictionary of molecular biology. 2004.

Look at other dictionaries:

  • Isoelectric focusing — (IEF), also known as electrofocusing, is a technique for separating different molecules by their electric charge differences. It is a type of zone electrophoresis, usually performed in a gel, that takes advantage of the fact that a molecule s… …   Wikipedia

  • isoelectric focusing — n an electrophoretic technique for separating proteins by causing them to migrate under the influence of an electric field through a medium (as a gel) having a pH gradient to locations with pH values corresponding to their isoelectric points * *… …   Medical dictionary

  • isoelectric focusing — isoelectric focusing. См. изоэлектрическое фокусирование. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • isoelectric focusing — noun Date: 1966 an electrophoretic technique for separating proteins by causing them to migrate under the influence of an electric field through a medium (as a gel) having a pH gradient to locations with pH values corresponding to their… …   New Collegiate Dictionary

  • isoelectric focusing — noun : an electrophoretic technique for separating proteins by causing them to migrate under the influence of an electric field through a medium (as a gel) having a pH gradient to locations with pH values corresponding to their isoelectric points …   Useful english dictionary

  • Isoelectric focusing — …   Википедия

  • isoelectric focusing — noun Biochemistry a technique of electrophoresis in which the resolution is improved by maintaining a pH gradient between the electrodes …   English new terms dictionary

  • Isoelectric point — The isoelectric point (pI) is the pH at which a particular molecule or surface carries no net electrical charge. Amphoteric molecules called zwitterions contain both positive and negative charges depending on the functional groups present in the… …   Wikipedia

  • isoelectric — Of equal electrical potential. Cf.:i. point. SYN: isopotential. i. focusing electrophoresis of small molecules or macromolecules in a pH gradient. * * * iso·elec·tric .ī sō i lek trik adj 1) having or representing the same or connecting points… …   Medical dictionary

  • IEF —    Isoelectric focusing …   Forensic science glossary

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