interference microscopy


interference microscopy
Although all image formation depends on interference, the term is generally restricted to systems in which contrast comes from the recombination of a reference beam with light that has been retarded by passing through the object. Because the phase retardation is a consequence of the difference in refractive index between specimen and medium, and because the the refractive increment is almost the same for all biological molecules, it is possible to measure the amount of dry mass per unit area of the specimen by measuring the phase retardation. Quantification of the phase retardation is usually done by using a compensator to reduce the bright object to darkness (see Senarmont and Ehringhaus compensators). Two major optical systems have been used - the Jamin-Lebedeff system and the Mach-Zehndersystem. These instruments are often referred to as interferometers, since they are designed for measuring phase retardation. Although their use has passed out of fashion, it may be that they will be employed more frequently in future in conjunction with image analysing systems.

Dictionary of molecular biology. 2004.

Look at other dictionaries:

  • Interference microscopy — involving measurements of differences in the path between two beams of light that have been split.[1] Types include: Classical interference microscopy Differential interference contrast microscopy Fluorescence interference contrast microscopy See …   Wikipedia

  • interference microscopy — noun A method of microscopy in which the phase changes caused by differences in the optical path within the specimen can be measured or made visible • • • Main Entry: ↑interfere * * * interference microscopy, the use of an interference microscope …   Useful english dictionary

  • Classical interference microscopy — (also referred to as quantitative interference microscopy) uses two separate light beams with much greater lateral separation than that used in phase contrast microscopy or in differential interference microscopy (DIC). In variants of the… …   Wikipedia

  • Microscopy — is the technical field of using microscopes to view samples and objects that cannot be seen with the unaided eye (objects that are not within the resolution range of the normal eye). There are three well known branches of microscopy, optical,… …   Wikipedia

  • Interference microscope — might refer to: * Classical interference microscopy (interference between well separated beams) * Differential interference contrast microscopy (also called Nomarski Interference Contrast or Nomarski microscopy) * Fluorescence interference… …   Wikipedia

  • interference microscope — interference microscopy. a microscope that utilizes light interference phenomena to create two superimposed images of an object, making possible the observation of transparent objects without using the staining technique. * * * interference… …   Useful english dictionary

  • interference microscope — interference microscopy. a microscope that utilizes light interference phenomena to create two superimposed images of an object, making possible the observation of transparent objects without using the staining technique. * * * …   Universalium

  • Interference lithography — (or holographic lithography) is a technique for patterning regular arrays of fine features, without the use of complex optical systems or photomasks. Basic principleThe basic principle is the same as in interferometry or holography. An… …   Wikipedia

  • Microscopy — The examination of minute objects by means of a microscope, an instrument which provides an enlarged image of an object not visible with the naked eye. Aside from the usual microscopy, there are various special types of microscopy including, for… …   Medical dictionary

  • interference reflection microscopy — An optical technique for detecting the topography of the side of a cell in contact with a planar substrate, and for providing information on the separation of the plasmalemma from the substrate. Interference between the reflections from the… …   Dictionary of molecular biology


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