insertional mutagenesis
Generally, mutagenesis of DNA by the insertion of one or more bases. Specific examples: (1) oncogenesis by insertion of a retrovirus adjacent to a cellular proto-oncogene;
(2) a strategy of mutagenesis with transposons. After a round of transposition, progeny are screened by PCR, with transposon- and gene-specific primers, for the proximity of the transposon sequence to the gene of interest. As PCR can only produce products up to 1-2 kb, a large fraction of progeny identified as positive by PCR will have a transposon close enough to the gene to inactivate or otherwise alter its pattern of expression.

Dictionary of molecular biology. 2004.

Look at other dictionaries:

  • Insertional mutagenesis — is mutagenesis of DNA by the insertion of one or more bases.[1] Insertional mutations can occur naturally, mediated by virus or transposon, or can be artificially created for research purposes in the lab. Contents 1 Signature tagged mutagenesis 2 …   Wikipedia

  • insertional mutagenesis — noun (genetics) a mutation caused by the insertion of exogenous DNA into a genome • Topics: ↑genetics, ↑genetic science • Hypernyms: ↑mutagenesis …   Useful english dictionary

  • viral-induced insertional mutagenesis — viral induced insertional mutagenesis. См. вирусный инсерционный мутагенез. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • Mutagenesis — (pronounced /mjuːtəˈdʒɛnɪsɪs/) is a process by which the genetic information of an organism is changed in a stable manner, resulting in a mutation. It may occur spontaneously in nature, or as a result of exposure to mutagens. It can also be… …   Wikipedia

  • mutagenesis — noun an event capable of causing a mutation • Hypernyms: ↑cause • Hyponyms: ↑insertional mutagenesis • Part Holonyms: ↑mutation, ↑genetic mutation, ↑chromosomal mutation …   Useful english dictionary

  • mutagenesis — 1. Production of a mutation. 2. Production of genetic alteration through use of chemicals or radiation. cassette m. the production of mutants within a region (often bounded by unique restriction sites) by the use of synthetic oligonucleotides… …   Medical dictionary

  • Signature tagged mutagenesis — Often abbreviated to STM, Signature Tagged Mutagenesis is a genetic technique used to study gene function. Recent advances in genome sequencing have allowed us to catalogue a large variety of organisms genomes, but the function of the genes they… …   Wikipedia

  • Knockout rats — Knockout rats=A knockout rat is a rat with a single gene disruption used for academic and pharmaceutical research. Knockout rats can mimic human diseases and are important tools for studying gene function (functional genomics) and for drug… …   Wikipedia

  • Transposable element — A bacterial DNA transposon Transposable elements (TEs) are sequences of DNA that can move or transpose themselves to new positions within the genome of a single cell. The mechanism of transposition can be either copy and paste or cut and paste .… …   Wikipedia

  • Gene therapy — using an Adenovirus vector. A new gene is inserted into an adenovirull. If the treatment is successful, the new gene will make a functional protein. Gene therapy is the insertion, alteration, or removal of genes within an individual s cells and… …   Wikipedia

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