- indirect immunofluorescence
A method of immunofluorescence staining in which the first antibody, that is directed against the antigen to be localized, is used unlabelled, and the location of the first antibody is then detected by use of a fluorescently labelled anti-IgG (against IgGs of the species in which the first antibody was raised). The advantage is that there is some amplification, and a well-characterized goat anti-rabbit IgG antibody can, for example, be used against a scarce specific antibody raised in rabbits. The same technique can be used for ultrastructural localization of the first antibody by substituting peroxidase or gold-labelled second antibody.
Dictionary of molecular biology. 2004.