in situ hybridization


in situ hybridization
(1) Technique for revealing patterns of gene expression in a tissue. The tissue is fixed and prepared (usually by sectioning) on a slide, and a labelled DNA or RNA probe is hybridized to the sample. It binds only to complementary mRNA sequences, and so only stains cells that are expressing the gene in question.
(2) Use of the technique for identifying the position of a gene on a chromosome by hybridizing a probe to spread chromosomes. Widely used in Drosophila salivary gland polytene chromosome "squashes", but now also possible with a variety of eukaryotic nuclei. ‘FISH’ analysis is the use of in situ hybridization.

Dictionary of molecular biology. 2004.

Look at other dictionaries:

  • In situ hybridization — (ISH) is a type of hybridization that uses a labeled complementary DNA or RNA strand (i.e., probe) to localize a specific DNA or RNA sequence in a portion or section of tissue ( in situ ), or, if the tissue is small enough (e.g. plant seeds,… …   Wikipedia

  • In situ hybridization — The use of a DNA or RNA probe to detect the complementary sequence. In situ hybridization is like all nucleic acid hybridization in being a technique in which single stranded nucleic acids (DNA or RNA) are permitted to interact so that complexes …   Medical dictionary

  • in situ hybridization — hibridizacija in situ statusas T sritis augalininkystė apibrėžtis Specifinių DNR sekų atpažinimo metodas sveikose chromosomose su radioaktyviu papildomuoju (komplementariuoju) zondu. atitikmenys: angl. in situ hybridization rus. гибридизация in… …   Žemės ūkio augalų selekcijos ir sėklininkystės terminų žodynas

  • in situ hybridization —  In situ hybridization  Гибридизация in situ   Метод, использующий одноцепочечный РНК или ДНК зонд для обнаружения гена или молекулы информационной РНК в клетке или ткани (см. также гибридизация) …   Толковый англо-русский словарь по нанотехнологии. - М.

  • in-situ hybridization — noun (biology) A technique, using a radioactive nucleic acid probe and autoradiography, for locating the position of a specific DNA sequence on a chromosome • • • Main Entry: ↑in situ …   Useful english dictionary

  • in situ hybridization — in situ hybridization. См. цитологическая гибридизация. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • in situ hybridization — The visualization of in vivo location of macromolecules (particularly polynucleotides and polypeptides) by the histological staining of tissue sections or cytological preparations via labelled probes/antibodies …   Glossary of Biotechnology

  • Fluorescence in situ hybridization — A metaphase cell positive for the bcr/abl rearrangement (associated with chronic myelogenous leukemia) using FISH. The chromosomes can be seen in blue. The chromosome that is labeled with green and red spots (upper left) is the one where the… …   Wikipedia

  • Fluorescent in situ hybridization — can be used to find out where the fluorescent probe bound to the chromosome. FISH is often used for finding specific features in DNA. These features can be used in genetic counseling, medicine, and species identification. Probes Probes are often… …   Wikipedia

  • Fluorescent in situ hybridization — Hybridation fluorescente in situ Pour les articles homonymes, voir FISH. Technique de l hybridation fluorescente in situ. En A : sonde. B : sonde colorée à l aide d un …   Wikipédia en Français


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