gel retardation assay


gel retardation assay
(= mobility shift assay)
Test for interaction between molecules by looking for a change in gel electrophoretic mobility. For example, to assay for levels of a transcription factor, cell extracts are incubated with a radiolabelled oligonucleotide corresponding to the recognition sequence of the transcription factor, and run on an agarose gel. Most of the radiolabel will run quickly through the gel, but any radioactivity that is retarded is presumably caused by DNA/transcription factor interaction.

Dictionary of molecular biology. 2004.

Look at other dictionaries:

  • gel retardation assay — an assay used to determine whether a specific protein binds to DNA or to determine some characteristics of such binding; protein is incubated with or without nucleic acid under various conditions and the electrophoretic mobilities of the samples… …   Medical dictionary

  • gel mobility shift — (= gel retardation assay) Technique for studying DNA protein interactions. For example, to study the levels of a particular transcription factor, nuclear protein extracts are incubated with a radiolabelled DNA fragment containing the… …   Dictionary of molecular biology

  • Electrophoretic mobility shift assay — An electrophoretic mobility shift assay (EMSA), also referred as a gel shift assay, gel mobility shift assay, band shift assay, or gel retardation assay, is a common technique used to study protein DNA or protein RNA interactions. This procedure… …   Wikipedia

  • electrophoretic mobility shift assay — gel retardation a …   Medical dictionary

  • гелевый электрофорез гель-э — гелевый электрофорез, гель э. * гелявы электрафарэз, гель э. * gel electrophoresis метод для разделения заряженных молекул в электрическом поле в гелевом матриксе на основе различий в электрическом заряде, форме и размерах молекул. Существует… …   Генетика. Энциклопедический словарь

  • Wound healing — Hand abrasion …   Wikipedia

  • analysis — /euh nal euh sis/, n., pl. analyses / seez /. 1. the separating of any material or abstract entity into its constituent elements (opposed to synthesis). 2. this process as a method of studying the nature of something or of determining its… …   Universalium

  • Guthrie test — The Guthrie test, also known as the Guthrie bacterial inhibition assay, is a medical test performed on newborn infants to detect phenylketonuria, an inborn error of amino acid metabolism. The test has been widely used throughout North America and …   Wikipedia

  • Life Sciences — ▪ 2009 Introduction Zoology       In 2008 several zoological studies provided new insights into how species life history traits (such as the timing of reproduction or the length of life of adult individuals) are derived in part as responses to… …   Universalium


Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”

We are using cookies for the best presentation of our site. Continuing to use this site, you agree with this.