gel filtration


gel filtration
An important method for separating molecules according to molecular size by percolating the solution through beads of solvent-permeated polymer that has pores of similar size to the solvent molecules. Unlike a continous filter that retards flow according to molecular size, separation is achieved because molecules that can enter the beads take a longer path (ie. are retarded) than those that cannot. Typical gels for protein separation are made from polyacrylamide, or from flexible (Sephadex) or rigid (agarose, Sepharose) sugar polymers. The size separation range is determined by the degree of cross-linking of the gel.

Dictionary of molecular biology. 2004.

Look at other dictionaries:

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  • gel filtration — noun : chromatography in which the material to be fractionated separates primarily according to molecular size as it moves through a column of a gel and is washed with a solvent so that the fractions appear successively at the end of the column… …   Useful english dictionary

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  • gel chromatography — gelchromatografija statusas T sritis Standartizacija ir metrologija apibrėžtis Chromatografija, kai molekulėms išskirstyti pagal matmenis vartojamas akytas polimero gelis. atitikmenys: angl. gel chromatography; gel filtration; gel permeation… …   Penkiakalbis aiškinamasis metrologijos terminų žodynas


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