functional cloning


functional cloning
(= expression cloning)
Strategy for cloning a desired gene that is based on some property (antigenicity, ligand binding, etc.) of the expressed gene. For example, a cDNA library could be produced in a eukaryotic expression vector, and transfected into a large number of cells. To identify a particular transport protein, a radiolabelled substrate could be added, and cells containing the protein of interest identified by radiography. The plasmid could then be recovered and the gene&’s sequence determined.

Dictionary of molecular biology. 2004.

Look at other dictionaries:

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