frozen stock


frozen stock
Because cell lines tend to change their properties with continuous rounds of subculturing, it is common practice to keep stocks of cells frozen (either in liquid nitrogen or at -70oC) and to keep returning to this stock so that experiments are all carried out on cells of comparable passage number. The method also allows strains to be stored for long periods. Cells are usually frozen down in the presence of a cryoprotectant such as DMSO or glycerol. The method is also extensively used for storing semen for artificial insemination.

Dictionary of molecular biology. 2004.

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