enhancer trap
Technique for mapping gene expression patterns, classically in Drosophila. A transposon element carrying a reporter gene (usually beta-galactosidase), linked to a very weak promoter, is induced to ‘jump’ within the genome. If the P-element re-inserts within the sphere of influence of promoters and enhancersof some (random) gene, then the reporter gene is also expressed in a similar tissue-specific manner. Usually, many lines of flies carrying such random insertions are studied; if a line shows "interesting" patterns of expression, it can be possible to clone the gene of interest.

Dictionary of molecular biology. 2004.

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  • Enhancer Trap — Das Enhancer Trap Verfahren ist eine molekularbiologische Methode, um transgene Organismen zu erzeugen, die ein Transgen nur in bestimmten Zellen exprimieren, ohne das es dabei nötig ist, Promotoren zu kennen, die für diesen Zelltyp spezifisch… …   Deutsch Wikipedia

  • Enhancer trap — In essence, enhancer traps are transgenic constructions by the fusing of two proteins that are inserted into the genome for the identification of enhancers. Its structure contains a mobile element (necessary for random insertion in the genome)… …   Wikipedia

  • GAL4 enhancer trap — Form of enhancer trap (classically in Drosophila) in which the reporter gene is the yeast transcription factor GAL4. The advantage of this system is that such enhancer traps can drive expression of any transgene under control of the UAS promoter… …   Dictionary of molecular biology

  • Enhancer (genetics) — In genetics, an enhancer is a short region of DNA that can be bound with proteins (namely, the trans acting factors, much like a set of transcription factors) to enhance transcription levels of genes (hence the name) in a gene cluster. While… …   Wikipedia

  • Gal4 — Schematischer Aufbau eines GAL4/UAS Systems Mit Hilfe des GAL4/UAS Systems (auch UAS GAL4 System) können beliebige klonierte Gene gezielt in bestimmten Zellen oder Geweben exprimiert werden.[1] GAL4 ist ein hefespezifischer Transkriptionsfaktor,… …   Deutsch Wikipedia

  • UAS-GAL4-System — Schematischer Aufbau eines GAL4/UAS Systems Mit Hilfe des GAL4/UAS Systems (auch UAS GAL4 System) können beliebige klonierte Gene gezielt in bestimmten Zellen oder Geweben exprimiert werden.[1] GAL4 ist ein hefespezifischer Transkriptionsfaktor,… …   Deutsch Wikipedia

  • P element — A P element is a transposon that is present specifically in the fruit fly Drosophila melanogaster and is used widely for mutagenesis and the creation of genetically modified flies used for genetic research. The P element gives rise to a phenotype …   Wikipedia

  • Spatiotemporal gene expression — is the activation of genes within specific tissues of an organism at specific times during development. Gene activation patterns vary widely in complexity. Some are straightforward and static, such as the pattern of tubulin, which is expressed in …   Wikipedia

  • List of genetics-related topics — This is a list of terms related to genetics. NOTOC # * 3 end * 5 end A * Acentric chromosome * Achondroplasia * Active site * Adam s Curse * Adaptation * Adenine * Adenosine * Adenovirus * Adenosine diphosphate (ADP) * Ala * Alagille syndrome *… …   Wikipedia

  • List of genetic engineering topics — NOTOC # 3 end 5 end A Acentric chromosome Achondroplasia Active site Adam s Curse Adaptation Adenine Adenosine Adenosine diphosphate Adenovirus ADP Ala Alagille syndrome Albino Alcoholism Alkylating agent Allele Allele frequency Alleles… …   Wikipedia

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