electrophoresis
Separation of molecules based on their mobility in an electric field. High resolution techniques normally use a gel support for the fluid phase. Examples of gels used are starch, acrylamide, agarose or mixtures of acrylamide and agarose. Frictional resistance produced by the support causes size, rather than charge alone, to become the major determinant of separation. The electrolyte may be continuous (a single buffer), or discontinuous, where a sample is stacked by means of a buffer discontinuity, before it enters the running gel/running buffer. The gel may be a single concentration or gradient in which pore size decreases with migration distance. In SDS gel electrophoresis of proteins or electrophoresis of polynucleotides, mobility depends primarily on size and is used to determined molecular weight. In pulse-field electrophoresis, two fields are applied alternately at right-angles to each other to minimize diffusion-mediated spread of large linear polymers. See also electrofocusing, pulse-field electrophoresis

Dictionary of molecular biology. 2004.

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  • Electrophoresis — is the most well known electrokinetic phenomenon. It was discovered by Reuss in 1809. [Reuss, F.F. Mem.Soc.Imperiale Naturalistes de Moscow , 2, 327 1809] He observed that clay particles dispersed in water migrate under influence of an applied… …   Wikipedia

  • electrophoresis — n. 1. (Chem.) the motion of charged molecules or particles in a liquid medium under the influence of an electric field; particles with a positive charge move toward the cathode and negative to the anode. [WordNet sense 1] Syn: cataphoresis.… …   The Collaborative International Dictionary of English

  • Electrophoresis —   Couverture actuelle Titre abrégé Electrophoresis …   Wikipédia en Français

  • electrophoresis — electrophoresis. См. электрофорез. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • Electrophoresis — Electrophoresis. См. Электрофорез. (Источник: «Металлы и сплавы. Справочник.» Под редакцией Ю.П. Солнцева; НПО Профессионал , НПО Мир и семья ; Санкт Петербург, 2003 г.) …   Словарь металлургических терминов

  • electrophoresis — [ē lek΄trōfə rē′sis, i lek΄trōfə rē′sis] n. [ModL < ELECTRO + (CATA)PHORESIS] the migration of charged colloidal particles or of molecules through a fluid or gel subjected to an electric field electrophoretic [ē lek΄trōfə ret′ik, i lek΄trōfə… …   English World dictionary

  • electrophoresis — A ubiquitous molecular biology technique, with many variants, used to resolve complex mixtures of macromolecules into their components. Its principle is to subject samples to an electric field applied across a porous matrix. Molecules will… …   Glossary of Biotechnology

  • electrophoresis — electrophoretic /i lek troh feuh ret ik/, adj. /i lek troh feuh ree sis/, n. 1. Also called cataphoresis. Physical Chem. the motion of colloidal particles suspended in a fluid medium, due to the influence of an electric field on the medium. 2.… …   Universalium

  • Electrophoresis — Method used in clinical and research laboratories for separating molecules according their size and electrical charge. Electrophoresis is used to separate large molecules (such as DNA fragments or proteins) from a mixture of molecules. An… …   Medical dictionary

  • electrophoresis — noun a) the migration of electrically charged molecules through a medium under the influence of an electric field b) a method for the separation and analysis of large molecules ( …   Wiktionary

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