DNA footprinting
Technique for identifying the recognition site of DNA-binding proteins: see footprinting.

Dictionary of molecular biology. 2004.

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  • DNA footprinting — is a method of investigating the sequence specificity of DNA binding proteins in vitro. This technique can be used to study protein DNA interactions both outside and within cells. The regulation of transcription has been studied extensively, and… …   Wikipedia

  • DNA-binding domain — A DNA binding domain (DBD) is an independently folded protein domain that contains at least one motif that recognizes double or single stranded DNA. A DBD can recognize a specific DNA sequence (a recognition sequence) or have a general affinity… …   Wikipedia

  • DNA binding site — DNA binding sites are a type of binding site found in DNA where other molecules may bind. DNA binding sites are distinct from other binding sites in that (1) they are part of a DNA sequence (e.g. a genome) and (2) they are bound by DNA binding… …   Wikipedia

  • footprinting — A method for determining the area of DNA covered by protein binding; accomplished by nuclease digestion of the protein DNA complex followed by analysis of the region of DNA protected by the interaction with protein. * * * foot·print·ing iŋ …   Medical dictionary

  • footprinting — A technique used to identify the binding site of, for example, a protein on a nucleic acid sequence. The basic principle is to carry out a very limited hydrolysis of the DNA with or without the protein complexed and then to compare the digestion… …   Dictionary of molecular biology

  • DNase footprinting assay — DNaseI footprint of a protein binding to a radiolabelled DNA fragment. Lanes GA and TC are Maxam Gilbert chemical sequencing lanes, see DNA Sequencing. The lane labelled control is for quality control purposes and contains the DNA fragment but… …   Wikipedia

  • Phylogenetic footprinting — is a technique used to identify transcription factor binding sites (TFBS) within a non coding region of DNA of interest by comparing it to the orthologous sequence in different species. When this technique is used with a large number of closely… …   Wikipedia

  • DNase Footprinting Assay — (DNase Fußabdruck Untersuchung) ist ein molekularbiologisches Verfahren zum Aufspüren von DNA Protein Interaktionen. Dabei wird die Tatsache ausgenutzt, dass DNA an Stellen, an denen ein Protein gebunden ist, zu einem gewissen Grad vor… …   Deutsch Wikipedia

  • DNAase Footprinting Assay — DNase Footprinting Assay (DNase Fußabdruck Untersuchung) ist ein molekularbiologisches Verfahren zum Aufspüren von DNA Protein Interaktionen. Dabei wird die Tatsache ausgenutzt, dass DNA an Stellen, an denen ein Protein gebunden ist, zu einem… …   Deutsch Wikipedia

  • Noncoding DNA — In genetics, noncoding DNA describes components of an organism s DNA sequences that do not encode for protein sequences. In many eukaryotes, a large percentage of an organism s total genome size is noncoding DNA, although the amount of noncoding… …   Wikipedia

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