Cot curve


Cot curve
Physicochemical technique for measuring the complexity (or size) of DNA. The DNA is heated to make it single stranded, then allowed to cool. The renaturation of the DNA is followed spectroscopically: larger DNA molecules take longer to reanneal.

Dictionary of molecular biology. 2004.

Look at other dictionaries:

  • cot curve — A method to estimate the heterogeneity of sequence of a DNA preparation, based on the observation that the more homogenous the DNA, the more easily (and therefore faster) the annealing of single stranded DNA will occur. The Cot curve plots the… …   Glossary of Biotechnology

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  • DNA annealing — The reformation of double stranded DNA from thermally denatured DNA. The rate of reassociation depends upon the degree of repetition, and is slowest for unique sequences (this is the basis of the Cot value; see Cot curve) …   Dictionary of molecular biology

  • reannealing — Renaturation of a DNA sample that has been dissociated by heating. In reannealing the two strands that recombine to form a double stranded molecule are from the same source. Differences in the rate of reannealing led to the early recognition of… …   Dictionary of molecular biology

  • repetitive DNA — Nucleotide sequences in DNA that are present in the genome as numerous copies. Originally identified by the value on the Cot curve derived from kinetic studies of DNA renaturation. These sequences are not thought to code for polypeptides. One… …   Dictionary of molecular biology

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